The Colony Forming Assay and colony counting is universally recognized as the gold standard method for measuring the effects of radiation, chemotherapeutic drugs and other agents on mammalian cell viability.
However, manually counting the resulting cell colonies, spheroids or organoids is a thankless, laborious and time-limiting task in which consistent objectivity is difficult to achieve.
GelCount™ is an easy-to-use, PC software-operated colony counter that automates the detection, counting and analysis of mammalian cell colonies in multi-well plates, Petri dishes and T25 flasks. Colonies and cell types can be either adherent (‘2D’), or non-adherent (‘3D’) seeded in semi-solid matrices or indeed suspension.
GelCount provides a powerful and cost-effective alternative to the subjective and labour-intensive task of manual colony counting in the colony forming cell assay, also referred to variously as the clonogenic assay, the cell survival assay, the tumour cloning assay, or the organoid formation assay.
The leading colony, spheroid and organoid counter for cancer biologists
GelCount™ is an all-in-one solution for imaging, counting and characterizing adherent or non-adherent colonies, spheroids or organoids on a single integrated hardware and accompanying proprietary PC software platform. Colonies are imaged, images transferred to a PC, images processed and characterized and the data collated/exported from within a single, integrated platform. Gone is the unsatisfactory alternative of imaging samples on one device, then transferring these to and processing them on a separate image analysis package.
Using single pass, high depth-of-field line imaging, combined with a single-axis motorized sample-carrier mechanism, GelCount provides unsurpassed colony detection performance including resolution of overlapping colonies and differentiation of real colonies from debris or other artefacts. Typically a set of four 6-well plates containing non-adherent colonies, spheroids or organoids can be imaged and processed in less than 15 minutes. Colonies as small as 30 µm in diameter are detectable across a medium depth (z-axis) of up to 5 mm (subject to contrast conditions).
Processing colony samples with the GelCount not only generates a numerical count but also yields colony diameter information in the form of a mean per well/dish, or a histogram distribution, or even on an individual colony basis if required. The ability to quantitatively measure the effects of anti-cancer therapeutic regimes not only on absolute colony numbers but also on colony size significantly extends the sensitivity of the colony forming assay by providing the user with hitherto unavailable information relating to colony and cell growth dynamics.
Our customer-focused service includes free and unlimited availability of software updates, fixes and enhancements for the lifetime of the product.
With a long-standing track record including 100’s of peer-reviewed citations and a worldwide user-base, the GelCount has become the solution of choice for biologists employing the colony / spheroid / organoid formation assay.
With GelCount the user goes from colony sample to colony counts, colony size distribution and a host of additional statistical data at the click of a button via an intuitive user interface. The image processing algorithm objectively applies user-definable colony detection parameters while distinguishing overlapping colonies and suppressing false positives. GelCount thereby not only dramatically enhances throughput but its inherent ‘machine’ objectivity and consistency eliminates human error due to subjective interpretation, bias or plain fatigue – a particularly acute problem when manually counting under a microscope.
Entire wells or Petri dishes can be conveniently viewed on-screen at high resolution.
GelCount can be used to count and analyze classic adherent colonies AND non-adherent colonies, organoids and spheroids in suspension or semi-solid 3D media, while supporting multi-well plates (up to 96-well), Petri dishes and select T25 flasks.
The software can be installed on unlimited other workstations. In this way images generated by GelCount can be stored, transferred and analyzed ‘offline’ at the user’s convenience, without tying up the imager for other users.
Colony / spheroid counts, diameter statistics and other numerical data are automatically exported to Excel®, while colony images can be saved in a raw format for subsequent offline processing or in a generic image format for printing, presentations, etc.