Vesi-Safe EV storage and handling buffer
Safely preserve your EV during storage
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Maintaining the stability of extracellular vesicles (EVs) is crucial for both storage and experimental procedures.
EVs are sensitive to various environmental factors, such as temperature fluctuations, pH changes, and freeze-thaw cycles. Vesi-Safe is a buffer specifically designed to stabilize EVs, helping to preserve their structural integrity and biological activity over time. This stability is essential for ensuring the reliability and reproducibility of experiments involving EVs.
Stability over 24 hours
Vesi-Ref CD63-mNG: properties/fluorescence
Analysis by Single EV Imaging Flow Cytometry (Cellstream)
Benefits of using Vesi-Safe:
- Prevents EVs from adhering to tube/plastics surfaces
- Experimentally tested over years
- Does not impede most analytics
- Can be used at 1x or 10x concentration to suit most applications
Commonly used buffers in EV research, such as PBS, lead to loss of material or to decreased EV recovery.
Vesi-Safe keeps EVs stable helps prevent these detrimental effects, ensuring that the EV population remains homogeneous and representative of the original sample. This is particularly important for longitudinal studies where samples are collected at different time points and need to be analysed under consistent conditions.
A Demonstrated Efficiency
Results shown/values given are as measured post dilution.
Stability in dilution
This data confirms that Vesi-Safe also for this batch prevents EV loss which occurs for EVs in PBS due to plastic adsorption
Perform assays without losing EVs to dilution
In experimental procedures, the stability of EVs is critical for accurate and reliable results. Fluctuations in EV integrity or recovery can affect their interactions with target cells or molecules, leading to inconsistent outcomes. Its superior stabilising properties allow Vesi-Safe buffer to maintain EV samples for extended periods of bench work without compromising their quality, enabling downstream analyses and future and cell-uptake/functional assays.
With Vesi-Safe, large samples numbers can safely be analysed in an automated way over long period of time, using flow cytometry for instance.
Preserve EVs straight after isolation
Stabilising extracellular vesicles immediately after isolation is crucial to maintain their integrity and functionality. EVs are highly sensitive to changes in their microenvironment, and their properties can rapidly deteriorate if not kept in the appropriate buffer.
PBS is commonly used in research for keeping EVs in a physiological strength environment straight after isolation, but it has been shown to be inadequate for both cryo-preservation, as well as for bench work. [Reference JEV article EV storage study]
Vesi-Safe buffer has been developed to offer the best performance for stabilising and preserving EVs for both storage and operational requirements. It can be used straight after most EV isolation techniques, either in it 10x form in a diluted sample, for example after gravity size exclusion chromatography, or diluted to 1x strength to resuspend UC pellets or TFF concentrates.
Enabling exosome biobanking
The need for a stabilising buffer for exosome biobanking arises from the inherent fragility of exosomes and the importance of preserving their integrity and functionality over extended periods of storage. EVs contain various bioactive molecules, including proteins, nucleic acids, and lipids, which are susceptible to degradation and changes in their microenvironment. Without proper stabilisation, exosomes stored in biobanks may undergo structural alterations, cargo loss, or aggregation, compromising their quality and biological activity.
Vesi-Safe buffer helps maintain the stability of exosomes by preserving their structural integrity, preventing degradation of cargo molecules, and minimizing the impact of storage-related factors such as temperature fluctuations and freeze-thaw cycles. By ensuring the long-term stability of exosomes, Vesi-Safe facilitates their use in downstream applications, including biomarker discovery, diagnostics, and therapeutics, thus advancing our understanding of exosome biology and their clinical potential.
Vesi-Ref CD63-mNG Technical Description
|
Volume |
Concentration |
Working Concentration |
Storage |
|
5 & 10ml |
10x |
1x |
Keep Refrigerated |
