Vesi-SEC midi
Spin Size Exclusion Chromatography (SEC) Columns for Rapid, High-Yield Isolation of Extracellular Vesicles and Viruses
Vesi-SEC Midi brings a new level of simplicity, speed, and reliability to the isolation of extracellular vesicles (EVs) and viruses from cell culture supernatants and biofluids. Engineered for performance and convenience, it combines the precision of size exclusion chromatography with the speed of spin-column formats, without compromising sample integrity.
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No Fractions, No Dilution
Sample load volume: 0.5–1 mL
Sample types: Cell culture media (including concentrated), urine, cerebrospinal fluid (CSF), plasma, serum, and other biofluids
Preparation time: 3 minutes
Operation time: 2 minutes
Scalability: Process 1 to 80 samples simultaneously (centrifuge-dependent)
Particle recovery: >90%
One-step recovery: No fractions. No dilutions. One single, purified sample.
Why Choose Vesi-SEC Midi?
Unlike traditional gravity-fed SEC columns that require tedious fraction collection and lead to sample dilution, Vesi-SEC Midi offers a streamlined, single-step workflow that’s ideal for high-throughput processing. Just load, spin, and collect.
- Gentle & unbiased: Separates EVs and viruses by size without altering their biological properties
- Efficient cut-off: The proprietary Core-Sep resin provides a 35 nm cut-off, effectively separating target particles from free proteins, lipid aggregates, dyes, and unbound affinity reagents
- Easy implementation: Compatible with standard low-speed centrifuges and 15 mL conical tubes using basic adaptors
- Preserves integrity: Optimized for high recovery without shear stress or chemical disruption
SP-IRIS analysis of cell-culture EVs isolated by Vesi-SEC Midi
|
CD9 EV concentration (particles/ml) |
CD63 EV concentration (particles/ml) |
CD81 EV concentration (particles/ml) |
|
3.23E+10 |
4.60E+10 |
6.10E+10 |
Recovery efficiency as measured by Interferometric Light Microscopy (ILM, Videodrop device)
SP-IRIS analysis of cell-culture EVs isolated by Vesi-SEC Midi
|
CD9 EV concentration (particles/ml) |
CD63 EV concentration (particles/ml) |
CD81 EV concentration (particles/ml) |
|
3.23E+10 |
4.60E+10 |
6.10E+10 |
Recovery efficiency as measured by Interferometric Light Microscopy (ILM, Videodrop device)
Vesi-SEC Midi is Perfect For:
- EV isolation from unprocessed or pre-concentrated culture supernatants
- Virus purification from complex fluids
- Downstream applications such as proteomics, RNA profiling, immunodetection, or functional assays
Vesi-SEC Midi makes EV and virus isolation faster, scalable, and lab-friendly, empowering you to focus on results, not protocol bottlenecks.
Vesi-SEC micro Technical Information
| Format | Spin columns containing 3mL Core-Sep gel |
| Sample capacity | 0.5ml-1.0mL |
| Quantity | 20 spin columns per box |
| Storage conditions | Up to 3 years at 4-6°C |
| Notes | For optimal performance, use swing-bucket rotor centrifuge |
